Turbonuclease

Ultra-pure version of Benzonase. Nonspecific endonuclease, hydrolyzes both single- and double-stranded nucleic acids (DNA and RNA) to 5’-phosphorylated oligonucleotides of 1-4 bases in length. MW = 27 kDa (homodimer). Expressed in E. coli. No detectable protease activity. TurboNuclease can be used to
• reduce viscosity of cell lysates
• reduce back pressure of column loading
• remove nucleic acid contamination from sample preparations
• reduce nucleic acid contamination of Ni column purification
• reduce smearing in SDS-PAGE when used with 10%SDS or Gel Loading Dye
• make whole cell lysates
• reduce or prevent clumping of concentrated cells and thawed cells
• replace crude DNase I in many applications

This product has been cited 8 times.