FBXL10 (KDM2B) Homogeneous Assay Kit

●Purchase●DescriptionTheFBXL10HomogeneousAssayKitisdesignedtomeasuretheactivityoftheFBXL10forscreeningandprofilingapplications

产品型号：50610
厂商性质：生产厂家
更新时间：2023-11-12
访问量：204

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Description

The FBXL10 Homogeneous Assay Kit is designed to measure the activity of the FBXL10 for screening and profiling applications. The key to the FBXL10 Homogeneous Assay Kit is a highly specific antibody that recognizes demethylated substrate. With this kit, only three simple steps on a microtiter plate are required for demethylase activity detection. First, a sample containing FBXL10 enzyme is incubated with the biotinylated substrate. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts.

Scheme 1: Our histone demethylase assays utilize highly specific antibodies that recognize demethylated products. First, a sample containing the enzyme is incubated with a biotinylated substrate. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts, as shown below.