TET2 Homogeneous Assay Kit

The TET2 (Tet Methylcytosine dioxygenase 2) Homogeneous Assay Kit is designed to measure the activity of TET2 for screening and profiling applications. The TET2 Homogeneous Assay Kit comes in a convenient AlphaLISA® format, with enough biotinylated TET2 substrate, TET2 assay buffer, and purified TET2 for 384 reactions.

The key to the TET2 Homogeneous Assay Kit is a highly specific antibody that recognizes the hydroxymethylated substrate, and the increase in Alpha-counts is proportional to the hydroxymethylation of the substrate. First, a sample containing TET2 enzyme is incubated with the reaction mixture. Next, acceptor beads are added, followed by donor beads and reading the Alpha-counts.