SetDB1 Chemiluminescent Assay Kit

The SetDB1 Chemiluminescent Assay Kit is designed to measure SetDB1 activity for screening and profiling applications. The SetDB1 Chemiluminescent Assay Kit comes in a convenient 96-well or 384-well format, precoated with histone H3 peptide substrate, an antibody against the methylated lysine 9 residue of Histone H3, a secondary HRP-labeled antibody, S-adenosylmethionine, methyltransferase assay buffer, and purified SetDB1 enzyme (amino acids 510-1290) for 96 or 384 enzyme reactions. The advantage of the SetDB1 Chemiluminescent Assay Kit is a highly specific antibody that recognizes the methylated K9 residue of Histone H3. With this kit, only three simple steps are required. First, S-adenosylmethionine is incubated with a sample containing the assay buffer and the methyltransferase enzyme. Next, the primary antibody is added. Finally, the wells are treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can be measured using a chemiluminescence reader

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