Growth Arrested PD-1 / NFAT Reporter Jurkat Cell Line

Growth Arrested PD-1 / NFAT Reporter Jurkat Cell Line is a Jurkat T cell cell line expressing firefly luciferase under the control of NFAT response elements with constitutive expression of human PD-1 (Programmed Cell Death 1, PDCD1, SLEB2, CD279, GenBank Accession #NM_005018).

Note: These cells are unable to complete mitosis and are suitable for single use assays. For proliferating cells, please use our PD-1 / NFAT Reporter Jurkat Cell Line (BPS Bioscience #60535).

Figure 1: Illustration of the mechanism of action of Growth Arrested PD-1 / NFAT Reporter Jurkat Cell Line in a co-culture assay.
The TCR activator present at the surface of PD-L1/TCR Activator CHO cells stimulate TCR in Jurkat T cells, whereas overexpression of PD-L1 on the CHO cell line engages Jurkat PD-1, blocking TCR activation signaling and preventing activation of NFAT. Addition of a neutralizing anti-PD-1 or anti-PD-L1 antibody to the co-culture releases the PD-L1/PD-1 complex and results in TCR activation and increased NFAT activity, which translates into increased luciferase reporter signal.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.