SiMPLe CHC Antibody Labeling Kit (Sortase Mediated Protein Ligation)

The SiMPLe CHC Antibody Labeling Kit leverages Sortase Mediated Protein Ligation to specifically label recombinant antibodies containing the sequence LPXTG at the C-terminus of the heavy chain (CHC). A diverse number of poly-Glycine (G)_n molecules and labels (e.g. fluorophores, biotin, enzymes, peptides, etc.) are compatible with this kit. The site-specific conjugation ensures that the antigen binding site remains available for target binding and reduces heterogeneity compared to chemical conjugation methods, making it ideal for Antibody-Drug Conjugate (ADC) development and flow cytometry. The kit provides reagents to label 3 x 100 μg of recombinant antibody, as well as purification columns to aid in the removal of excess poly-Glycine (G)_n-label. Anti-HER2-LPETGH₆ and GGGK-FITC are included as positive controls.

Figure 1: Illustration of the mechanism of targeted labeling using the SiMPLe CHC Antibody Labeling Kit (Sortase Mediated Protein Ligation).
Each antibody contains two Sortase recognition sequences, so a maximum of two labels per antibody can be achieved