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CRISPRa (SAM) HEK293 Cell Line

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  • 厂商性质:生产厂家
  • 更新时间:2023-10-11
  • 访  问  量:202
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详细介绍
  • 数量:

    1

  • 供应商:

    BPS Bioscience

  • 细胞类型:

    稳转细胞系

  • 品系:

    HEK293

  • 组织来源:

    /

  • 相关疾病:

    多种

  • 物种来源:

    human

  • 免疫类型:

    多种

  • 器官来源:

  • 运输方式:

    干冰

  • 年限:

    10

  • 生长状态:

    良好

  • CAS号:

  • 英文名:

    CRISPRa (SAM) HEK293 Cell Line

  • 注册证号:

    /

  • 规格:

    2管

This cell line has been engineered for use with the CRISPR Synergistic Activation Mediator (SAM) system to induce transcriptional activation and expression of any gene of interest. Cells stably express a mutated dCas9 (Streptococcus pyogenes CRISPR associated protein 9), lacking any endonuclease activity, fused to transcriptional activator VP64. Stable dCas9-VP64 expression is maintained with Blasticidin resistance. Cells also stably express P65 (Transcription Factor p65, or Nuclear Factor NF-κB p65) and HSF1 (Heat Shock Factor 1) fused with an MS2 tag, which is maintained with Hygromycin resistance. When these cells are transfected with an MS2- tagged sgRNA targeting the promoter region of the gene of interest, dCas9-VP64 and MS2-P65-HSF1 are recruited to the genomic DNA and begin transcription, inducing expression of the desired gene.

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