Human CD226 antigen also known as DNAX accessory molecule 1 (DNAM-1), GenBank Accession No. NM_006566, a.a. 19-247 fused to Fc region of human IgG1, expressed in a HEK293 cell expression system. MW = 52.8 kDa (monomer). This protein runs at a higher MW by SDS-PAGE due to glycosylation.